2.3.2 - Plant sample oxidation

Plant studies with ¹⁴C are typically performed by growing the plant with ¹⁴C labelled carbon dioxide. Distribution of the ¹⁴C is then defined by analysing samples from different parts of the plant.

Procedure

1. Plant sample chopping into a ceramic sample boat. Typically, 50-300 mg of plant.

2. Weight measurement of the plant sample

3. The ceramic sample boats and empty LSC vials are placed into 600 OX Oxidizer

4. Combustion time of 3 minutes is selected

5. Start the automated combustion

6. After combustion, cap the LSC vials and measure the ¹⁴C activity with a LSC instrument